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bigdye ® terminator v3.1 cycle sequencing chemistry  (Thermo Fisher)


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    Thermo Fisher bigdye ® terminator v3.1 cycle sequencing chemistry
    Bigdye ® Terminator V3.1 Cycle Sequencing Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bigdye+terminator+cycle+sequencing+chemistry/bigdye+terminator+v3+1+cycle+sequencing+kit/pmc11856360-133-7-14
    Average 90 stars, based on 1 article reviews
    bigdye ® terminator v3.1 cycle sequencing chemistry - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Biallelic variants in HTRA2 cause 3-methylglutaconic aciduria mitochondrial disorder: case report and literature review.
    Article Snippet: .. Variants were confirmed by Sanger sequencing of PCRamplified products from the proband and negative control reference samples using BigDye Terminator cycle sequencing chemistry (Applied Biosystems) on an ABI 3730xl Genetic Analyzer. ..

    Article Title: Mutations in LRP5 cause primary osteoporosis without features of OI by reducing Wnt signaling activity
    Article Snippet: .. Mutation analysis was performed with direct sequencing using the ABI PRISM ® 3100 Genetic Analyzer and BigDye terminator cycle sequencing chemistry (Applied Biosystems). ..

    Article Title: Molecular Identification, Phylogenetic Status, and Geographic Distribution of Culicoides oxystoma (Diptera: Ceratopogonidae) in Israel
    Article Snippet: Inserts containing plasmids were isolated with the DNA-spinTM kit (Intron Biotechnology, Kyungki-Do, Korea). .. DNA sequencing was carried out utilizing the BigDye Terminator cycle sequencing chemistry from Applied Biosystems (ABI, Carlsbad, CA, USA), the 3700 DNA analyzer, and the ABI data-collection and sequence-analysis software (Center for Genomic Technologies, The Hebrew University of Jerusalem). .. Sequences were analyzed using DNAMAN (Lynnon Corporation, Pointe-Claire, Canada) and deposited in GenBank.

    Article Title: Responses of potential ammonia oxidation and ammonia oxidizers community to arsenic stress in seven types of soil.
    Article Snippet: Soil arsenic contamination is of great concern because of its toxicity to human, crops, and soil microorganisms.. However, the impacts of arsenic on soil ammonia oxidizers communities remain unclear.. Seven types of soil spiked with 0 or 100 mg arsenic per kg soil were incubated for 180 days and sampled at days 1, 15, 30, 90 and 180.

    Article Title: A mutagenesis-derived Lrp5 mouse mutant with abnormal retinal vasculature and low bone mineral density
    Article Snippet: .. PCR products were purified using QIAquick PCR purification according to the manufacturer’s recommendations (QIAGEN, Germantown, MD) and sequenced using BigDye Terminator Cycle Sequencing Chemistry and separated by capillary electrophoresis on Applied Biosystems 3730xl DNA Analyzer. .. RNA was isolated from whole-eyes enucleated from P8 animals using an RNeasy Mini kit (QIAGEN).

    Article Title: Borrelia persica infection in wild carnivores in Israel: molecular characterization and new potential reservoirs
    Article Snippet: .. Sequencing was performed by the BigDye Terminator cycle sequencing chemistry from Applied Biosystems ABI 3700 DNA Analyzer (ABI, Carlsbad, CA, USA) and the ABI Data collection and Sequence Analysis software at the Center for Genomic Technologies, Hebrew University of Jerusalem, Israel. ..

    Article Title: Novel Missense Mutation in GJB1 Gene Leading to X-linked Charcot–Marie–Tooth Disease in Young Male: A Case Report
    Article Snippet: Charcot–Marie–Tooth disease (CMT) is a genetically heterogeneous disorder.. As more genes are identified and overlapping of neuropathy phenotypes, the traditional classification of CMT often proves inadequate.. A young male with typical clinical features was suspected to have CMT, but family history about the inheritance pattern and distinct features suggestive of particular subtypes were lacking.

    Negative Control:

    Article Title: Biallelic variants in HTRA2 cause 3-methylglutaconic aciduria mitochondrial disorder: case report and literature review.
    Article Snippet: .. Variants were confirmed by Sanger sequencing of PCRamplified products from the proband and negative control reference samples using BigDye Terminator cycle sequencing chemistry (Applied Biosystems) on an ABI 3730xl Genetic Analyzer. ..

    Mutagenesis:

    Article Title: Mutations in LRP5 cause primary osteoporosis without features of OI by reducing Wnt signaling activity
    Article Snippet: .. Mutation analysis was performed with direct sequencing using the ABI PRISM ® 3100 Genetic Analyzer and BigDye terminator cycle sequencing chemistry (Applied Biosystems). ..

    DNA Sequencing:

    Article Title: Molecular Identification, Phylogenetic Status, and Geographic Distribution of Culicoides oxystoma (Diptera: Ceratopogonidae) in Israel
    Article Snippet: Inserts containing plasmids were isolated with the DNA-spinTM kit (Intron Biotechnology, Kyungki-Do, Korea). .. DNA sequencing was carried out utilizing the BigDye Terminator cycle sequencing chemistry from Applied Biosystems (ABI, Carlsbad, CA, USA), the 3700 DNA analyzer, and the ABI data-collection and sequence-analysis software (Center for Genomic Technologies, The Hebrew University of Jerusalem). .. Sequences were analyzed using DNAMAN (Lynnon Corporation, Pointe-Claire, Canada) and deposited in GenBank.

    Software:

    Article Title: Molecular Identification, Phylogenetic Status, and Geographic Distribution of Culicoides oxystoma (Diptera: Ceratopogonidae) in Israel
    Article Snippet: Inserts containing plasmids were isolated with the DNA-spinTM kit (Intron Biotechnology, Kyungki-Do, Korea). .. DNA sequencing was carried out utilizing the BigDye Terminator cycle sequencing chemistry from Applied Biosystems (ABI, Carlsbad, CA, USA), the 3700 DNA analyzer, and the ABI data-collection and sequence-analysis software (Center for Genomic Technologies, The Hebrew University of Jerusalem). .. Sequences were analyzed using DNAMAN (Lynnon Corporation, Pointe-Claire, Canada) and deposited in GenBank.

    Article Title: Borrelia persica infection in wild carnivores in Israel: molecular characterization and new potential reservoirs.
    Article Snippet: .. Sequencing was performed by the BigDye Terminator cycle sequencing chemistry from Applied Biosystems ABI 3700 DNA Analyzer (ABI, Carlsbad, CA, USA) and the ABI Data collection and Sequence Analysis software at the Center for Genomic Technologies, Hebrew University of Jerusalem, Israel. ..

    Article Title: Borrelia persica infection in wild carnivores in Israel: molecular characterization and new potential reservoirs
    Article Snippet: .. Sequencing was performed by the BigDye Terminator cycle sequencing chemistry from Applied Biosystems ABI 3700 DNA Analyzer (ABI, Carlsbad, CA, USA) and the ABI Data collection and Sequence Analysis software at the Center for Genomic Technologies, Hebrew University of Jerusalem, Israel. ..

    Clone Assay:

    Article Title: Responses of potential ammonia oxidation and ammonia oxidizers community to arsenic stress in seven types of soil.
    Article Snippet: Soil arsenic contamination is of great concern because of its toxicity to human, crops, and soil microorganisms.. However, the impacts of arsenic on soil ammonia oxidizers communities remain unclear.. Seven types of soil spiked with 0 or 100 mg arsenic per kg soil were incubated for 180 days and sampled at days 1, 15, 30, 90 and 180.

    Polymerase Chain Reaction:

    Article Title: A mutagenesis-derived Lrp5 mouse mutant with abnormal retinal vasculature and low bone mineral density
    Article Snippet: .. PCR products were purified using QIAquick PCR purification according to the manufacturer’s recommendations (QIAGEN, Germantown, MD) and sequenced using BigDye Terminator Cycle Sequencing Chemistry and separated by capillary electrophoresis on Applied Biosystems 3730xl DNA Analyzer. .. RNA was isolated from whole-eyes enucleated from P8 animals using an RNeasy Mini kit (QIAGEN).

    Article Title: Novel Missense Mutation in GJB1 Gene Leading to X-linked Charcot–Marie–Tooth Disease in Young Male: A Case Report
    Article Snippet: Charcot–Marie–Tooth disease (CMT) is a genetically heterogeneous disorder.. As more genes are identified and overlapping of neuropathy phenotypes, the traditional classification of CMT often proves inadequate.. A young male with typical clinical features was suspected to have CMT, but family history about the inheritance pattern and distinct features suggestive of particular subtypes were lacking.

    Purification:

    Article Title: A mutagenesis-derived Lrp5 mouse mutant with abnormal retinal vasculature and low bone mineral density
    Article Snippet: .. PCR products were purified using QIAquick PCR purification according to the manufacturer’s recommendations (QIAGEN, Germantown, MD) and sequenced using BigDye Terminator Cycle Sequencing Chemistry and separated by capillary electrophoresis on Applied Biosystems 3730xl DNA Analyzer. .. RNA was isolated from whole-eyes enucleated from P8 animals using an RNeasy Mini kit (QIAGEN).

    Electrophoresis:

    Article Title: A mutagenesis-derived Lrp5 mouse mutant with abnormal retinal vasculature and low bone mineral density
    Article Snippet: .. PCR products were purified using QIAquick PCR purification according to the manufacturer’s recommendations (QIAGEN, Germantown, MD) and sequenced using BigDye Terminator Cycle Sequencing Chemistry and separated by capillary electrophoresis on Applied Biosystems 3730xl DNA Analyzer. .. RNA was isolated from whole-eyes enucleated from P8 animals using an RNeasy Mini kit (QIAGEN).



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